https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46776 Wed 30 Nov 2022 13:21:41 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37216 Tue 01 Sep 2020 14:51:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41755 86%) significantly decreasing compared to control conditions. Additionally, almost 60% of the coral volatilome (or 52 BVOCs) could be assigned to four key functional groups based on their activities in other species or systems, including stress response, chemical signalling, climate regulation and antimicrobial activity. The total number of compounds assigned to these functions decreased significantly under heat stress for both A. intermedia (by 35%) and P. damicornis (by 64%), with most dramatic losses found for climatically active BVOCs in P. damicornis and antimicrobial BVOCs in A. intermedia. Together, our observations suggest that future heat stress events predicted for coral reefs will reduce the diversity, quantity and functional potential of BVOCs emitted by reef-building corals, potentially further compromising the healthy functioning of these ecosystems]]> Thu 24 Aug 2023 15:48:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46512 Thu 16 May 2024 10:32:29 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42705 Thu 01 Sep 2022 09:55:19 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50775 Sat 05 Aug 2023 10:06:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48524 Acropora muricata High light (HL)- and low light (LL)-acclimated corals were collected from the reef and reciprocally exposed to high and low light ex situ Rapid light curves using pulse-amplitude modulation (PAM) fluorometry revealed photophysiological acclimation of LL corals to HL and HL corals to LL within 21 days. A subset of colonies sampled at 7 and 21 days for untargeted LC-MS and GC-MS metabolomic profiling revealed metabolic reorganization before acclimation was detected using PAM fluorometry. Metabolomic shifts were more pronounced for LL to HL corals than for their HL to LL counterparts. Compounds driving metabolomic separation between HL-exposed and LL control colonies included amino acids, organic acids, fatty acids and sterols. Reduced glycerol and campesterol suggest decreased translocation of photosynthetic products from symbiont to host in LL to HL corals, with concurrent increases in fatty acid abundance indicating reliance on stored lipids for energy. We discuss how these data provide novel insight into environmental regulation of metabolism and implications for management strategies that drive rapid changes in light availability.]]> Mon 20 Mar 2023 17:56:36 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46262 Symbiodinium tridacnidorum and Durusdinium trenchii) using gas chromatography–mass spectrometry (GC–MS) and comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC x GC–TOFMS). Seven chemical classes were detected by both instruments, the most common being aromatic hydrocarbons. However, GC x GC resolved seven times more BVOCs than GC–MS (290 vs. 40), with a higher proportion of compounds tentatively identified (173 vs. 14). Notably, nine chemical classes were exclusively identified by GC x GC, including alkane, alkene, aldehyde, ester, and nitrile BVOCs—each potentially fulfilling undescribed functions in marine organisms. The microalgal species investigated shared a large proportion of BVOCs, and this result was consistent across instruments (97 and 98% shared compounds via GC x GC and GC–MS, respectively), suggesting consistent retrieval of general patterns between instruments. This method comparison is the first of its kind in marine systems and confirms the greater analytical power of GC x GC, required to help resolve complex BVOC emissions and the identification of their roles in marine systems.]]> Mon 14 Nov 2022 15:58:32 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41225 −1) with no evident pattern with algal phylogeny. Intriguingly, net carbon uptake rates (24 h) were often higher (1.01–5.54 pg C [cell h]⁻¹) than corresponding values of GPC—we discuss how such GPC measurements may reflect highly conserved biological characteristics for cultured cells linked to high metabolic dependency on photorespiration and heterotrophy. Three isolates from different genera (Cladocopium goreaui, Durusdinium trenchii, and Effrenium voratum) were additionally grown at 20°C and 30°C. Here, Ci uptake consistently decreased with temperature-driven declines in growth rate, suggesting environmental regulation outweighs phylogenetic organization of carbon assimilation capacity among Symbiodiniaceae. Together, these data demonstrate environmental regulation and ecological success among Symbiodiniaceae likely rests on plasticity of upstream photosynthetic processes (light harvesting, energy quenching, etc.) to overcome evolutionary-conserved limitations in Ci functioning.]]> Fri 29 Jul 2022 11:06:39 AEST ]]>